. Author manuscript; available in PMC: 2024 Apr 24.

Published in final edited form as: J Am Chem Soc. 2011 Aug 9;133(34):13232–13235. doi: 10.1021/ja204249p

Table 2.

Kinetic parameters for Ag85 isoforms recorded under pseudo-single substrate conditions.

Ag85 Isoform	Varied Substrate^a	K_M(app) (μM)	k_cat(app) (s⁻¹)	k_cat(app)/K_M(app) (M⁻¹s ¹)
a	1	175	0.014	82
b	1	112	0.003	30
c	1	62	0.182	2952

^a:

Assay conditions: [TDH]=500 μM, [trehalose]=10-250 μM, [Ag85a/b]=500 nM, [Ag85c]=50 nM.