Figure 3. Enhancement of nucleosome mechanical stability by DNA mismatch.

Average of FRET vs. Force for ED1 probe (A) and ED2 probe (B) for the 601 nucleosome (black) and for the first stretching cycle of the mismatch containing nucleosome 601-R39 (purple). Histone proteins were expressed in Xenopus. The error bars represent S.D. of n=25 and 7 for the ED1 probe of the 601 and 601-R39 nucleosomes (A) and n=20 and 39 for the ED2 probe of the 601 and 601-R39 nucleosomes (B), respectively.