Figure 7. Unwrapping of yeast vs. Xenopus reconstituted nucleosomes.

Average of FRET vs. Force for nucleosomes reconstituted from Xenopus (red) vs yeast (black and gray) histone proteins with DNA labeled by outer turn probes ED1 (A), ED2 (B) and inner turn probe INT (C). The error bars represent S.D. of n=17 (Xenopus) and 5 (Yeast) nucleosomes with the ED1 probe (A), n=20 (Xenopus), 6 (Yeast – strong) and 4 (Yeast-weak) nucleosomes with the ED2 probe (B), and n=22 (Xenopus) and 6 (Yeast) nucleosomes with the INT probe (C), respectively.