FIG. 3.

Representation of luciferase activity in 293 cells after cotransfection and nuclear comicroinjection with EBNA1 expression plasmid pTG11149 together with pTG11056 (oriP+) or pTG11077 (oriP−). A total of 3 × 10⁴ 293 cells were seeded per well on 48-well plates. The next day, the cells were transfected with 30 ng of pTG11149 together with 30 ng of pTG11077 or pTG11056. Alternatively, a solution of 10 ng of pTG11149 per μl together with 10 ng of pTG11056 or pTG11077 per μl was injected into the nuclei of 100 293 cells. At 16 h after cotransfection or comicroinjection, the cells were harvested and total cell protein was prepared. One-fifth of the material was analyzed with respect to luciferase activity. The obtained RLU are indicated.