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. 2024 Apr 24;81(1):196. doi: 10.1007/s00018-024-05239-7

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Global transcriptomic changes after telomerase modulation in CRISPRi TERT hiPSCs. A PCA plot indicates the distance between CRISPRi TERT hiPSC samples. X and Y axis show principal component 1 and principal component 2 that explain 70% and 17% of the total variance, respectively. (n = 4 per condition) CRISPRi TERT hiPSCs long (− Doxycycline, C, black circles), intermediate (+ Doxycycline, P2, yellow circles), short telomeres (+ Doxycycline, P5, red circles) and the reverse group (doxycycline removed for 2 weeks from P5 samples, R, green circles) (Supplemental Table 1 for list of top 2000 highly expressed genes) B Heatmap illustrates the differential gene expression of telomerase and telomere shelterin proteins between CRISPRi TERT hiPSCs under different conditions (x-axis: C, P2, P5 and R) (n = 4 /condition) in red: upregulation, in blue: downregulation C Bubble plots of functional enrichment analysis. The plot shows selected Gene Ontology Biological Processes enriched after treatment with doxycycline 5 passages. (Supplemental Table 2 for whole functional enrichment analysis). D Gene Ontology (GO) analysis of differentially regulated genes shows overrepresented biological process between R and P5 as depicted in bubble plots. Bubble size represents the ratio between the genes connected to a process and the overall query size. The color scale shows the adjusted p-value (Supplemental Table 3 for whole functional enrichment analysis)