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. 2024 Apr 24;81(1):196. doi: 10.1007/s00018-024-05239-7

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Mesodermal differentiation is influenced by telomerase activity of the stem cells. A Identification of the 10 clusters after single cell RNA sequencing of day 12 differentiated cells arising from CRISPRi TERT hiPSCs with long (− Doxycycline) and short (+ Doxycycline for passage 2 and passage 5) telomere lengths, n = > 4500 cells/group. B Gene expression profiles (columns) of selected 6 cell type-specific markers (well known from literature) used for categorizing each cluster into a particular mesodermal cell type as shown in the dot plot. Bar on the right displays the percentage of total dataset represented in every cluster, showing the abundance of each cell type found by clustering analysis. C Venn diagram depicting top 50 genes of the 5 main cell types (from the total 10 clusters). Only 1 gene overlaps between Endothelial and Cardiomyocyte cell cluster. D Distribution of the cells arising from differentiation of CRISPRi TERT hiPSCs with long telomeres (− Doxycycline, dark grey) and intermediate telomere length (two passage + Doxycycline, yellow) and short telomere length (five passage + Doxycycline, red) across the different clusters. Black arrows highlight shift in the cell distribution. E Abundance of cells in specific clusters arising from the various groups of the doxycycline induced CRISPRi TERT hiPSCs