FIG. 4.
The antibody (2D7) to CCR5 is able to block productive infection of thymocytes by JR-CSF. Thymocytes were preincubated in the presence or absence of antibody to CCR5 (2D7) or CXCR4 (12G5) for 2 h before infection with JR-CSF (30 IU/104 cells). The antibodies were present during the infection and throughout the culture with IL-2 plus IL-4. HIV replication was detected by measuring p24 antigen in the culture supernatants on days 8, 15, and 22 postinfection. Preincubation conditions: no antibody (gray bars), 1 μg of 2D7 (vertically striped bars), 5 μg of 2D7 (diagonally striped bars), 10 μg of 12G5 (horizontally striped bars), 1 μg of 2D7 plus 10 μg of 12G5 (checkered bars), 5 μg of 2D7 plus 10 μg of 12G5 (black bars), and 100 μg of CD4-IgG (white bars).