Fig. 6. Peptides inhibit ECM protein secretion by TGFβ-activated healthy and scleroderma fibroblasts.

A Western blot of supernatants from fibroblasts incubated for 20 h in the absence of serum without (Ctrl) or with 40 μM each of P2 + P5 and without or with 10 ng/ml TGFβ. Peptide treatment strongly reduced amounts of secreted (pro)collagen I (top), fibrillin1 (mid) and fibronectin (bottom) in fibroblasts from healthy donors and scleroderma patients before and particularly after stimulation of ECM production by TGFβ. B Quantification of (A). N = 3 independent samples. Data are shown as mean ± SD.