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. 2024 Apr 24;14:9458. doi: 10.1038/s41598-024-60049-y

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Experimental detection of the distribution of LOY in sorted blood cells from patients with primary CRC, liver metastases of CRC and controls. (A) Distribution of LOY in five subpopulations of CD45 + blood leukocytes. LOY was analyzed in sorted B cells, Gran, Mono, NK, Tregs obtained from 49 male CRC patients, 13 LM_CRC patients and 19 healthy controls. CRC colorectal cancer, LM_CRC liver metastasis of CRC, Ctrl control patients. Pairwise comparison (Wilcoxon test with Bonferroni correction) is shown only for Tregs vs. B cells and Tregs vs. NK cells. These subsets (B cells and NK cells) displayed the highest statistical significance upon comparison with Tregs. (B) Distribution of LOY in FACS sorted subpopulations of T lymphocytes. Analysis of the distribution of LOY in Tregs, Th and CTLs in blood samples collected from 13 LM_CRC patients.