Figure 3.

Effect of ihfA deletion combined with increased bglG expression on Bgl⁺ mutations. (A) Effect of deleting ihfA on total Bgl⁺ mutations in the cells over expressing bglG. (B) Effect of deleting ihfA on insertional Bgl⁺ mutations in the cells over expressing bglG. Bgl⁺ mutation assays were performed as previously described. Two bglG overexpression strains, Ptet-G (Ptet driving bglG at the intS locus) and Iq-G (lacIq driving bglG at the intS locus), were used together with or without the ihfA deletion. This experiment serves to determine if the ihfA deletion has similar inhibitory impact on IS1/IS5 insertions in the cells with higher levels of BglG. Our previous study showed that more BglG produced leads to greater insertional frequencies (Zhang et al., 2022). Colony PCR was used to differentiate insertional and non-insertional Bgl⁺ mutants.