Figure 4.

Overexpression of ihfA and ihfB on a plasmid slightly lowers the IS insertional rate into bgl. (A) Effect of IhfA and IhfB overproduction on total Bgl⁺ mutations. (B) Effect of IhfA and IhfB overproduction on insertional Bgl⁺ mutations. (C) Effect of titrating ihfA and ihfB expressions on a plasmid on total Bgl⁺ mutations. (D) Effect of titrating ihfA and ihfB expressions on a plasmid on insertional Bgl⁺ mutations. For (C,D), mutation assays were performed as previously described on M9 salicin plates containing aTC at 0 to 30 ng/mL over a 10-day period. 0aTC = ●; 5aTC = ▲; 15aTC = ■; 20aTC = ∆; 30aTC = ○.