FIG. 1.

The substrates for site-specific integration. (A) Schematic representation of the AAV genome; (B) schematic representation of the EBV-based shuttle vector with the AAVS1 insert of 0.51 or 1.6 kb. The AAVS1 sequences show the putative TRS and an RBS. BamHI and HindIII were the restriction sites used for cloning the AAVS1 inserts. The genes of the vector backbone in which the recombinants had deletions are indicated; hyg^R, hygromycin resistance gene; EBNA-1, EBV-encoded nuclear antigen; oriP, EBV origin; ITR, inverted terminal repeat.