TABLE 1.
Summary of the deletions of AAV, AAVS1, and vector backbone sequences found in the recombinants
| Recombinant (AAVS1)a | AAV deletion (nt) | AAVS1 deletion upstream of RBS | AAVS1 deletion downstream of RBS (nt) | 220.2 deletion (nt) |
|---|---|---|---|---|
| R19 (1.6) | 288– 1351 | 824–1603 | 1– 88; 5988– 8952 | |
| R24 (1.6) | 1– 82; 4595– 4680 | 824– 1603 | 1– 88; 5988– 8952 | |
| R27 (1.6) | 288– 3105 | 1– 386 | 820– 1603 | 1– 88; 101– 520; 5988– 8952 |
| R35 (1.6) | 1– 3171; 4598– 4680 | 645– 1603 | 1– 88; 4667– 8952 | |
| R39 (0.51) | 1– 3818; 4547– 4680 | 1– 387 | 101– 786; 4713– 8539 |
a
Recombinants R19, R24, R27, and R35 were isolated from the same cell line carrying the 1.6-kb AAVS1 sequence. Recombinants R19 and R24, R27, and R35 were retrieved after AAV infection of the cell line at passages 10, 11, and 21, respectively. Recombinant R39 was isolated from a cell line carrying the 0.51-kb AAVS1 sequence. Numbers in parentheses are sizes in kilobases.