TABLE 2.
Comparison of the lengths of deleted episome sequences and the lengths of inserted sequencesa
| Recom- binant | Original size of episome (kb) | Size of recom- binant (kb) | Length of deletion (kb) | Length of insertion (kb) | Length of inserted AAV (kb) |
|---|---|---|---|---|---|
| R19 | 10.5 | 16.5 | 3.8 | 9.8 | 3.5 |
| R24 | 10.5 | 12.7 | 3.8 | 6.0 | 4.5 |
| R27 | 10.5 | 7.6 | 4.6 | 1.7 | 1.7 |
| R35 | 10.5 | 6.6 | 5.3 | 1.4 | 1.4 |
| R39 | 9.5 | 5.5 | 4.7 | 0.7 | 0.7 |
a
On account of the inherent instability of the AAVS1-shuttle vector, it was not possible to distinguish between deletions occurring prior to or during the AAV integration event.