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Smoke/aerosolization and sample conditions used with the SAEIVS for the high content screening experiments for the ALI exposure of BEAS-2B cell to derive a MEC.
| Test article | Puff interval/volume/profile | Dilution factor 1/x | Number of sticks/devices per run | Runs | Puffs per run | Final puff number applied ( a ) |
|---|---|---|---|---|---|---|
| 1R6F | 30 s/55 mL/bell shape/vent block | 5 | 3 | 1 | 7 (1.4 a ) | 7 (1.4 a ) |
| HTP | 30 s/55 mL/bell shape/no vent block | 1 | 3 | 5 | 8 | 40 |
The Smoke machine was used according to ISO, 20768.
