TABLE 5.

Central composite design and responses of TRS (mg/g) with different biomass loading (%) and enzyme units U).

Run order	Biomass loading (%)	Enzyme units U)	Total reducing sugar (mg/g)
1	3.5 (0)	40 (0)	339.49
2	1.37 (−alpha)	40 (0)	719.22
3	3.5 (0)	40 (0)	360.74
4	2 (−1)	60 (+1)	540.08
5	3.5 (0)	40 (0)	343.76
6	3.5 (0)	68.28 (+alpha)	294.90
7	5 (+1)	20 (−1)	94.76
8	3.5 (0)	11.715 (−alpha)	209.70
9	5.62 (+alpha)	40 (0)	94.87
10	3.5 (0)	40 (0)	365.46
11	3.5 (0)	40 (0)	355.96
12	5 (+1)	60 (+1)	143.66
13	2 (−1)	20 (−1)	496.46