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Analysis of extrachromosomal Hirt preparation DNA from cells exposed to virus. Cells were exposed to either SSAV or GALV-S for 18 h prior to isolation of low-molecular-weight DNA (see Materials and Methods). Digested and undigested DNAs were hybridized to a 32P-labeled BamHI-to-PstI DNA fragment derived from the GALV-S envelope coding region. Extrachromosomal DNA was digested with enzymes predicted to produce a 9.0-kb linear viral DNA band in infected cells.
