Figure 6.

Tumor suppression is mediated by CD8+T cells. (A) MC38.OVA tumor-bearing mice were immunized with transduced DCs or by direct injection with lentiviral vector (CD40L or PD-1.CD40L.OVA1/OVA2). Seven days postimmunization, TILs were separated into CD8+ and CD8− populations on magnetic beads and injected intrathecally into tumor-bearing mice. The CD8 population was 95% pure, while the CD8− population was 99% pure. (B) After adoptive transfer of CD8+ and CD8− TILs, tumor size (n=5) was monitored. *p≤0.05, ***p≤0.001. DCs, dendritic cells; TILs, tumor-infiltrating lymphocytes.