Figure 5.

Characteristics of genes contributing highly to an accurate CD8 immunophenotype classification. (A) The frequency and magnitude of every feature in the RNA-seq classifier was measured by training and validating a classifier 100 times on random subsets of the data. The frequency of every feature and its median magnitude are displayed. Genes used in >85% of the classifiers are labeled. (B) Heatmap showing the relative expression of top classification-relevant genes across CD8 immunophenotype class cancer types as well as in (C) CRC and lung cancer single-cell RNA-seq data and (D) bulk RNA-seq data. *p≤0.05. **p≤0.01. ***p≤0.001. ****p≤0.0001. CRC, colorectal cancer; IHC, immunohistochemistry; NSCLC, non-small-cell lung cancer; LN, lymph nodes; mets, metastases; ns, not significant; RNA-seq, RNA-sequencing.