FIG. 11.

Binding kinetics of gp64 glycosylation site mutants. Equivalent titers of wild-type AcMNPV (open circles) or recombinant baculoviruses encoding mutant gp64s lacking various consensus N-glycosylation sites (closed circles, AcSD64ΔN198; open boxes, AcSD64ΔN198,355; closed boxes, AcSD64ΔN198,355,385; open triangles, AcSD64ΔN355,385,426) were added to triplicate cultures of Sf9 cells and incubated for various times at 4°C. The viral inocula were then removed, the cells were washed three times with growth medium, and an agarose overlay was added. After 1 to 2 weeks at 28°C, plaques were counted under a dissecting microscope and the average numbers of plaques obtained with each virus were plotted against binding time.