FIG. 3.

Transient expression of gp64 N-glycosylation site mutants. Sf9 cells were transfected with plasmids encoding wild-type (WT) or mutant gp64s lacking various consensus N-glycosylation sites, as indicated above the lanes. The cells were treated with 0.2 mM castanospermine from 16 to 20 h and labeled with 100 μCi of Tran³⁵S-label per ml of radiolabeling medium containing 0.2 mM castanospermine from 20 to 24 h posttransfection. At the end of the labeling period, intracellular gp64 was extracted and immunoprecipitated, and the washed immunoprecipitates were analyzed by SDS-PAGE and autoradiography.