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. 1998 Dec;72(12):9459–9469. doi: 10.1128/jvi.72.12.9459-9469.1998

FIG. 6.

FIG. 6

Carbohydrate compositions of individual N-linked glycans on AcMNPV gp64. Sf9 cells were infected at a multiplicity of about 0.01 PFU per cell with wild-type AcMNPV (WT) or recombinant baculoviruses encoding mutant gp64s with single N-glycosylation sites at position 198, 355, 385, or 426, as indicated above the lanes. BV progeny were partially purified and solubilized, total virion proteins were resolved by SDS-PAGE, and the proteins were then transferred to Immobilon filters and probed with various digoxigenylated lectins, all as described in Materials and Methods. Each lectin (ConA [A], AAA [B], RCA [C], or SNA [D]) was preincubated in buffer alone (−) or buffer containing excess competing sugar (+) prior to use, and lectin binding was detected with alkaline phosphatase-conjugated sheep antidigoxigenin and a standard color reaction (2). Control strips were probed with a monoclonal antibody against gp64 (Ab) followed by alkaline phosphatase-conjugated secondary antibody and the same color reaction. Arrows at the left and right of each panel indicate the positions of wild-type (w) and mutant (m) gp64s.