FIG. 8.

Cell surface expression of gp64 with various glycosylation site mutations. Sf9 cells were infected at a multiplicity of about 2.5 PFU per cell with wild-type AcMNPV (WT) or recombinant baculoviruses encoding mutant gp64s lacking various consensus N-glycosylation sites, as indicated above the lanes. The cells were incubated to 40 h postinfection, radioiodinated by the lactoperoxidase method, and extracted. The extracts were immunoprecipitated with the control antibody PAb419 (lanes a) or with AcV1 (lanes b), and the immunoprecipitates were washed, disrupted, and analyzed by SDS-PAGE and autoradiography.