Fig. 7.

Cytokine and chemokine production of MRC-5 fibroblast before and after RV infection. (A) IL-6, (B) CXCL8, (C) VEGF, (D) CXCL10, (E) IL-1β. MRC-5 fibroblast cells were treated with Bifidobacterium animalis subsp. lactis Bl-04 (25 bacteria/cell) for 24 h and baseline samples (0 h) were collected. Remaining wells were infected with low (3 experiments), medium (2 experiments) or high titer (1 experiment) of RV for 2 h. Bl-04 treated and control samples were collected after 24, 48 and 72 h of incubation and cytokines were analyzed by ELISA. Error bars represent the SD of three replicate wells. Statistically significant result of Bl-04 versus control is marked with an asterisk (IL-1β high titer 24 h p = 0.037). ND = not detected.