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. 2024 Mar 25;9(16):17891–17902. doi: 10.1021/acsomega.3c08877

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© 2024 The Authors. Published by American Chemical Society

Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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Biological evaluation of hydrogels. (a) Cell proliferation assay by PrestoBlue after 7 days post cell seeding: (i) MC3T3-E1 and (ii) hBMSCs. (b) Mineralization of hydrogels with alizarin red staining of (i) 7 days post cell seeding with MC3T3-E1 on hydrogels, (ii) 7 days post cell seeding with hBMSCs on hydrogels, (iii) 14 days post cell seeding with MC3T3-E1 on hydrogels, (iv) 14 days post cell seeding with hBMSCs on hydrogels, (v) 21 days post cell seeding with MC3T3-E1 on hydrogels, and (vi) 21 days post cell seeding with hBMSCs on hydrogels. (0, 1, 5, and 10 attached to the nomenclature represent the weight % of mineral inducers added in that particular composition).