FIGURE 11.

Mobilization of neutrophils and M1 macrophages in the peripheral blood and in the stroke area was observed as early as 3 hrs. Whereas other cell types such as NK cells and macrophages (F4/80+, which also contain M2 type macrophages) gradually increased in the stroke areas. Following collection of peripheral blood from each stroke animal, animals were euthanized and perfused with ice cold PBS and the brain tissues from stroke area were collected and single cell suspensions were made for flow cytometry. * = significant differences