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Methods to load cargo inside exosomes. (A) Genetic engineering methods to load exosomes with protein and nucleic acid. MS2CP, MS2 coat protein; MBS, MS2 binding site; CRY2, cryptochrome 2; CIBN, truncated version of CRY-interaction basicloop- helix 1 protein; NLS, nuclear localization signal; BASP1, Brain Abundant Membrane Attached Signal Protein 1; HuR, Human Antigen R. (B) Physical methods to load proteins and nucleic acids into exosomes
