FIG. 1.

Cell surface radioimmunoassay of the expression of epitope 190/4 in AGMK cell lines. Duplicate wells of GL37, BS-C-1, and CV-1 cells grown in 96-well plates were treated with dilutions of MAb 190/4 or anti-human fibronectin MAb FN-15 ascites fluid, washed extensively, and treated with ¹²⁵I-labeled sheep anti-mouse Ab. After being washed extensively, the plates were autoradiographed at −70°C for 48 h with an intensifying screen.