FIG. 7.

p53 only modestly affects replication of the E1B 55-kDa mutant virus in H1299-p53 cells. Portions of H1299-p53 and parental H1299 cells were shifted to 32°C or maintained at 39°C. Twelve hours later, cells maintained at 39°C or shifted to 32°C were infected with either the wild-type virus dl309 or the E1B 55-kDa mutant viruses dl338 and dl1520 at an MOI of 9 PFU per cell. Virus yields were measured by plaque assay on 293 cells, using lysates harvested at 48 h postinfection for cells infected at 39°C and 4 days postinfection for cells infected at 32°C. Virus yields are expressed as average PFU per cell derived from two independent experiments performed in duplicate; error bars indicate standard errors of the means.