FIG. 8.

H1299 cells infected with the E1B 55-kDa mutant virus synthesize wild-type levels of late viral proteins when infected and maintained at 39°C. Parental H1299 cells were shifted from 37 to 39 or 32°C for 24 h and then mock infected or infected with either the wild-type virus dl309 or the E1B 55-kDa mutant virus dl1520 at an MOI of 30. Cells were labeled with ³⁵S-labeled amino acids for 1 h at 32 h postinfection for cells maintained at 39°C or at 64 h postinfection for cells maintained at 32°C. Proteins from 10⁵ cells (per lane) were separated by SDS-PAGE. Five viral late proteins were visualized and quantified with a PhosphorImager. The positions of migration and masses (in kilodaltons) of molecular weight standards are indicated on the right. The positions of five Ad late proteins were determined by using Ad virion standards labeled with ¹⁴C-amino acids; these proteins are identified on the left. Assoc, associated.