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. 2024 Apr 24;22:383. doi: 10.1186/s12967-024-05183-x

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — AZGP1 does not affect cell migration or invasion in PCa cells. A Wound-healing assay of PC3 vector control and PC3 AZGP1-OV cells shows no effect on cell migration. C Quantification of migration in PC3 vector control and PC3 AZGP1-OV cells. B Western blot confirming the overexpression of AZGP1 in PC3 AZGP1-OV cells. D Wound-healing assay of DU145 vector control and DU145 AZGP1-OV demonstrating no effect of AZGOP1 on DU145 cell migration. F Quantification of migration ability in DU145 vector control and DU145 AZGP1-OV cells. E Western blot confirming the overexpression of AZGP1 in DU145 AZGP1-OV cells. G Transwell migration assay PC3 vector control and PC3 AZGP1-OV demonstrating does not affect PC3 cell migration. H Quantification of migration ability in PC3 vector control and PC3 AZGP1-OV cells. I Transwell invasion assay of PC3 vector control and DU145 AZGP1-OV showing that overexpression of AZGP1 does not affect PC3 cell invasion. J Quantification of invasion ability in PC3 control and AZGP1-OV cells