Fig. 7.

AZGP1 is involved in angiogenesis in human prostate cancer. A Low expression level of AZGP1 is correlated with poor survival in the PRAD-TCGA database. B Lower expression of AZGP1 is associated with higher Gleason scores in the PRAD-TCGA database. C Decreased expression level of AZGP1 shows strong correlation with poor survival in the MSKCC database. D Lower expression of AZGP1 is associated with higher Gleason scores in the MSKCC database. E GO biological process analysis of the 74 overlapping genes shows significant roles in angiogenesis. F GO molecular function analysis of the 74 overlapping genes. TF: transcription factor G WikiPathways analysis of the 74 genes implicates the VEGF signaling pathway as the possible target of AZGP1 expression modulation in prostate cancer cells. H Representative images of immunofluorescence staining of DAPI, AZGP1, and CD31 in a human tissue microarray (TMA) of localized prostate cancer. I Representative image of a virtual hematoxylin and eosin stain of TMA cores to demonstrate the cancer morphology with superimposed microvessels determined by AI. J Human prostate tumor samples were divided at the median into high and low blood vessel density. AZGP1 expression was significantly higher in the low blood vessel density group compared to the high blood vessel density group. K The expression of AZGP1 is negatively correlated with blood vessel density