Figure 1.

Corneal hypoxia develops in a topical inoculation model of A. fumigatus keratitis. Hypoxyprobe was injected intraperitoneally 90 minutes before harvesting eyes infected with A. fumigatus at the indicated time points (n = 2 per group). Control and sham-infected animals were similarly injected with Hypoxyprobe and taken at 24 hours p.i. Ocular sections were stained with DAPI as a control (blue) along with anti-Hypoxyprobe antibodies to detect Hypoxyprobe bound to proteins (red). Isotype controls demonstrating stain specificity can be observed in Supplementary Figure S1. A serial section was stained with periodic acid Schiff and hematoxylin (PASH) as a reference and to visualize A. fumigatus hyphae (fuchsia). Scale bars are 100 µm. All animals in this experiment were male.