Figure 2.

Hif1α and glycolytic gene expression is elevated in A. fumigatus infected corneas. A. fumigatus (n = 6) or sham-infected corneas (n = 4) were collected at 24 hours p.i. along with untouched corneas as controls (n = 4). Following RNA isolation and first-strand cDNA synthesis, qRT-PCR was used to measure relative expression of the indicated genes with actin serving as a reference to plot the relative fold change 2^(-ΔΔCT); error bars reflect the standard deviation of the mean. For statistical analysis, the average ∆Cts of the groups were analyzed in a One-way ANOVA *P < 0.05, **P < 0.01. All animals in this experiment were male.