Figure 3.

Hypoxia development is driven primarily by the host response to fungal antigen. A. fumigatus conidia were swollen in YPD and half of the inoculum was killed with 70% ethanol. Corneas were ulcerated and overlaid with either the live conidia (n = 3), killed conidia (n = 4), PBS as a sham (n = 3), or left untouched (n = 3). Hypoxyprobe was injected intraperitoneally 90 minutes prior to euthanization and harvesting of eyes at 6 hours p.i. Ocular sections were stained with DAPI as a control (blue) along with anti-Hypoxyprobe antibodies to detect Hypoxyprobe bound to proteins (red). A serial section was stained with periodic acid Schiff and hematoxylin (PASH) as a reference and to visualize A. fumigatus hyphae (fuchsia). Scale bars are 100 µm. All animals in this experiment were male.