Extended Data Fig. 8 |. TAZ mutants lacking the CC domain still bind to LAST2 and TeAD4.

a, HA-tagged WT and mutant TAZ were co-transfected into 293T cells with Flag-LATS2. TAZ proteins associated with LATS2 were isolated by immunoprecipitation with anti-Flag and detected by western blotting with anti-HA antibodies (upper panels). The abundance of these proteins in the cell lysates was assessed by western blotting (lower panels). GApDH was used as a loading control. b, Interaction of various TAZ mutants with Flag-TEAD4 was analyzed by co-Ip assay as described in a. c, Interaction of various TAZ/YAp chimera with LATS2 was analyzed by co-Ip as described in a. All experiments were repeated independently three times with similar results. Unprocessed blots are provided in Unprocessed Blots Extended Data Fig. 8.