FIG. 7.
Time course of antibody response in sera of mice deficient or not for TNF-α/LTα. Sera of TNF-α/LTα+/+ (white bars), TNF-α/LTα+/− (gray bars), and TNF-α/LTα−/− (black bars) mice were obtained at different days (11, 29, 95, and 162) after the first adenovirus administration (5 × 109 PFU of Adα1hAT) and at 15 days (day 177) after the second adenovirus administration (8.8 × 109 PFU of AdRSVβgal). The sera were tested for the presence of IgG+IgM antibodies (upper panel) or IgM (lower panel). The values correspond to the mean (n = 3) of relative amounts (OD490) of antibodies in sera at dilutions of 1:4,000 (A1), 1:32,000 (A2), and 1:500 (B). Accurate estimation of antibody levels at day 177 was made on sera at a dilution of 1:32,000 in order to be within the linear range of the ELISA (A2). The standard deviations are shown.