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A) Overview of the development cohort. A total of 185 colorectal cancer (CRC) samples were analyzed. Forty of these samples were tested on a total of three extraction method and two different real-time PCR instruments. Thirty of the samples were analyzed in a total of five laboratories (1; Locarno, 2; Legnano, 3; Novara, 4; Candiolo, and 5; Terni). B) The validation cohort covered 127 CRC samples distributed in four laboratories.
