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. 2024 Apr 25;19(4):e0302274. doi: 10.1371/journal.pone.0302274

Fig 2. Principle of MicroSight® MSI analysis using universal reference.

Fig 2

Four stable and four unstable tumor samples were analyzed using a universal reference for NR24 locus. Applying a temperature shift makes it possible to differentiate unstable from stable samples naturally differing in lengths. A) Normalized HRM curves without temperature shift. B) Difference plot without temperature shift. Threshold was set at -0.04 RFU. C) Normalized HRM curves with temperature shift at 0.1 RFU. D) Difference plot with temperature shift at 0.1 RFU. Threshold was set at -0.04 RFU. MSI: microsatellite instability; HRM: high resolution melt; RFU: relative fluorescence unit.