Table 1. MicroSight® MSI agreement to the PCR fragment analysis.
| Type | Extraction method | PCR Instrument | Laboratory | MSS (agreement%) | MSI-L (agreement %) | MSI-H (agreement %) | Cohen’s Kappa [95% CI] |
|---|---|---|---|---|---|---|---|
| Paired | QIAamp | MyGo Pro | 1 | 151/151 (100) | 1/1 (100) | 33/33 (100) | 1.000 |
| Paired | BasePurifier | MyGo Pro | 1 | 13/13 (100) | 0/0 (100) | 27/27 (100) | 1.000 |
| Paired | BaseRelease | MyGo Pro | 1 | 13/13 (100) | 0/0 (100) | 27/27 (100) | 1.000 |
| Paired | BasePurifier | BaseTyper | 1 | 13/13 (100) | 0/0 (100) | 27/27 (100) | 1.000 |
| Paired | QIAamp | MyGo Pro | 2 | 25/25 (100) | 0/0 (100) | 5/5 (100) | 1.000 |
| Paired | QIAamp | MyGo Pro | 3 | 25/25 (100) | 0/0 (100) | 5/5 (100) | 1.000 |
| Paired | QIAamp | MyGo Pro | 4 | 25/25 (100) | 0/0 (100) | 5/5 (100) | 1.000 |
| Paired | QIAamp | MyGo Pro | 5 | 25/25 (100) | 0/0 (100) | 5/5 (100) | 1.000 |
| Universal | QIAamp | MyGo Pro | 1 | 151/151 (100) | - | 33/33 (100) | 1.000 |
| Universal | BasePurifier | MyGo Pro | 1 | 13/13 (100) | - | 27/27 (100) | 1.000 |
| Universal | BaseRelease | MyGo Pro | 1 | 12/13 (92.3) | - | 27/27 (100) | 0.9419 [0.8295; 1.000] |
| Universal | BasePurifier | BaseTyper | 1 | 13/13 (100) | - | 27/27 (100) | 1.000 |
| Universal | QIAamp | MyGo Pro | 2 | 25/25 (100) | - | 5/5 (100) | 1.000 |
| Universal | QIAamp | MyGo Pro | 3 | 25/25 (100) | - | 5/5 (100) | 1.000 |
| Universal | QIAamp | MyGo Pro | 4 | 25/25 (100) | - | 5/5 (100) | 1.000 |
| Universal | QIAamp | MyGo Pro | 5 | 25/25 (100) | - | 5/5 (100) | 1.000 |