Figure 4. Female and male wildtype (WT) osteocyte transcriptomes are distinctly different; however, female and male knockout (KO) osteocyte transcriptomes have fewer differences compared to WT female and male transcriptomes.

(A) Volcano plot showing the significantly regulated genes between WT female control (WT F) and WT male control (WT M) osteocyte transcriptome. (B) Volcano plot showing the significantly regulated genes between KO female control (KO F) and KO male control (KO M) osteocyte transcriptome. (C) Volcano plot showing the significantly regulated genes between WT male control (WT M) and KO male control (KO M) osteocyte transcriptome. (D) Volcano plot showing the significantly regulated genes between WT female control (WT F) and KO female control (KO F) osteocyte transcriptome. (E) Heat map showing the differentially expressed genes (DEG) among WT female control (WT F), WT male control (WT M), KO female control (KO F), and KO male control (KO M) osteocyte transcriptome. (F) Gene set enrichment analysis of gene ontology (GO) analysis of the significantly regulated genes between WT female control (WT F) and WT male control (WT M) osteocyte transcriptome, between KO female control (KO F) and KO male control (KO M) osteocyte transcriptome, WT male control (WT M) and KO male control (KO M) osteocyte transcriptome, and WT female control (WT F) and KO female control (KO F) osteocyte transcriptome. The figure shows the union of the top 10 GO terms of each analysis. If a term in the union, besides the top 10, is also significant (adjusted p-value less than 0.05 was used for GO analysis) in an analysis, it is also included in the figure. The latter group in the figure’s title is the reference group. n = 3/group. For DEG analysis, unadjusted p-value <0.01 was used.