FIG. 4.

Mta expression increased cytoplasmic accumulation of five EBV replication gene mRNAs. HeLa cells were transfected with the original expression vector for either BMRF1, BALF2, BSLF1, BBLF4, BALF5, or BBLF2/3 as indicated and cotransfected with either control (−) or Mta-expressing (+) plasmid. Cytoplasmic RNA was isolated and subjected to Northern analysis for expression of the indicated message. Both genomic (BBLF2/3) and cDNA (BBLF2/3 cDNA) versions of BBLF2/3 were tested. Mta had no effect on BBLF2/3 message, which was found to be constitutively expressed in the cytoplasm in the absence of Mta. In contrast, the other replication mRNAs were undetectable in the cytoplasmic fraction in the absence of Mta (−) and accumulated in the presence of Mta (+).