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Mta colocalizes with the SC35 splicing factor. Flag-tagged Mta was transfected into Vero cells. The Mta-expressing cells were fixed and immunostained with anti-Flag (rabbit polyclonal) and anti-SG35 (mouse monoclonal) primary antibodies. Secondary antibodies were FITC-conjugated anti-rabbit and TRITC-conjugated anti-mouse. Mta expression was diffusely nuclear, with a concentration in subnuclear regions which overlap with SC35 speckles.
