Figure 5:

Pro-inflammatory and anti-inflammatory macrophages are significantly impacted by CD4⁺ or CD8⁺ T cell deficiency. (A) Schematic demonstrating a co-culture model of macrophages with T cells. (B) Flow cytometry analysis of macrophages cultured in direct contact with T cells from either WT, Cd4^−/−, or Cd8^−/− mice. Cells were co-cultured on either TCPS, smooth Ti, rough Ti, or rough hydrophilic Ti for 48 hours (n=6). p<0.05: A vs. WT, B vs. Cd4^−/−, # vs. TCPS, $ vs. smooth Ti, % vs. rough Ti.