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. 2024 Apr 18;15(4):363–378. doi: 10.5312/wjo.v15.i4.363

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©The Author(s) 2024. Published by Baishideng Publishing Group Inc. All rights reserved.

This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial.

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Exercise promotes osteogenic differentiation of mouse bone mesenchymal stem cells. A: Alkaline phase staining of mouse bone mesenchymal stem cells (BMSCs) at 7 d of osteogenic differentiation induction; B: Alizarin red staining of mouse BMSCs at 14 d of osteogenic differentiation induction; C and D: Identification of potential miRNAs targets of lncRNA H19 by bioinformatics analysis; E and F: Changes in mRNA levels of lncRNA H19, miR-149, and osteogenic differentiation-associated markers after induction of osteogenic differentiation in mouse primary BMSCs by RT-qPCR; G and H: Changes in protein level of osteogenic differentiation-related marker and autophagy-related marker after induction of osteogenic differentiation in mouse primary BMSCs. n = 3, ^aP < 0.05, ^bP < 0.01. Con: Control group; OI: Osteogenic differentiation induction; Ex: Treadmill exercise group.