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. 2024 Apr 18;15(4):363–378. doi: 10.5312/wjo.v15.i4.363

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©The Author(s) 2024. Published by Baishideng Publishing Group Inc. All rights reserved.

This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial.

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Exercise promotes osteogenic differentiation of bone mesenchymal stem cells through upregulation of lncRNA H19/miR-149. A: Alkaline phase staining of bone mesenchymal stem cells (BMSCs) 3 d after osteogenic induction of differentiation; B: Alizarin red staining of BMSCs 3 d after osteogenic induction of differentiation; C: Gene expression changes of lncRNA H19, miR-149 and osteogenic differentiation-related markers in BMSCs after osteogenic differentiation induction and si-lncRNA H19 interference by RT-qPCR; D and E: Protein expression changes of osteogenic differentiation-associated markers and autophagy-associated markers in BMSCs after osteogenic differentiation induction and si-lncRNA H19 interference by WB technique. n = 3, ^aP < 0.05, ^bP < 0.01. Con: Control group; NC: Negative control group; OI: Osteogenic differentiation induction group.