Table 2.
Genes
|
Primer sequence (5'-3')
|
lncRNA H19 | Forward: 5'- GCTCCACTGACCTTCTAAAC -3' Reverse: 5'- ACGATGTCTCCTTTGCTAAC -3' |
miR-149 | Forward: 5'- TGGCTCCGTATCTTCACTCC -3' |
β-actin | Forward: 5'-CTGTCCCTGTATGCCTCTG-3' Reverse: 5'-ATGTCACGCACGATTTCC-3' |
ALP | Forward: 5'-CCAACTCTTTTGTGCCAGAGA-3' Reverse: 5'-GGCTACATTGGTGTTGAGCTTTT-3' |
Runx2 | Forward: 5'-AGAGTCAGATTACAGATCCCAGG-3' Reverse: 5'-TGGCTCTTCTTACTGAGAGAGG-3' |
BMP2 | Forward: 5'- GTGCAGATCCTAGGTTTCTCTG-3' Reverse: 5’- CAGGATCTCATTCTCTTGGATC-3’ |
Osterix | Forward: 5'-TGGTACAAGGCAGGCATCCA-3' Reverse: 5'-GGAGCAAAGTCAGATGGGTAAGT-3' |