. 2024 Mar 22;17(3):672–681. doi: 10.14202/vetworld.2024.672-681

Table-3.

Total-VFA and Individual VFA (mM) of experimental diets.

Time	Total-VFA

	111	112	113	121	122	123	211	212	213	221	222	223
6 h	101.68	101.7	100.41	101.28	101.26	100.88	102.12	102.36	102.83	102.93	102.27	102.21
24 h	107.59	108.16	105.96	108.55	108.3	107.85	106.81	105.9	106.34	106.21	105.95	106.2
48 h	117.31	116.88	116.53	116.42	116.52	118.77	114.82	114.73	114.77	114.41	113.43	113.72

Time	Acetate

	111	112	113	121	122	123	211	212	213	221	222	223

6 h	23.03	23.19	22.52	23.11	23.1	22.73	24.24	24.32	24.56	24.69	24.45	24.14
24 h	27.07	27.35	25.35	27.35	27.31	27.21	26.07	25.83	25.72	25.72	25.26	25.49
48 h	31.8	31.16	31.56	31.28	31.48	32.37	29.22	29.62	28.36	29.76	28.51	28.84

Time	Propionate

	111	112	113	121	122	123	211	212	213	221	222	223

6 h	17.58	17.33	16.88	17.13	17.35	17.27	17.07	17.16	17.38	17.23	16.8	16.83
24 h	18.87	19.31	19.28	19.53	19.51	18.95	19.06	18.39	18.95	18.23	18.85	18.85
48 h	20.92	21.19	20.43	20.5	20.38	21.35	20.5	20.68	20.91	19.95	20.23	20.23

Time	Butyrate

	111	112	113	121	122	123	211	212	213	221	222	223

6 h	15.27	15.3	15.29	15.3	15.24	15.26	15.23	15.25	15.26	15.41	15.41	15.39
24 h	15.71^a	15.63^ab	15.46^bc	15.50^bc	15.47^bc	15.50^bc	15.53^bc	15.52^bc	15.51^bc	15.45^bc	15.42^c	15.41^c
48 h	17.07	17.09	17.06	17.08	17.11	17.69	17.62	17.38	17.94	17.53	17.46	17.43

Time	Iso-butyrate

	111	112	113	121	122	123	211	212	213	221	222	223

6 h	15.26	15.28	15.26	15.26	15.20	15.23	15.20	15.23	15.22	15.22	15.22	15.23
24 h	15.47	15.39	15.34	15.40	15.36	15.40	15.40	15.40	15.40	15.46	15.41	15.45
48 h	15.81	15.78	15.79	15.84	15.84	15.76	15.78	15.66	15.82	15.72	15.77	15.76

Time	Iso-valerate

	111	112	113	121	122	123	211	212	213	221	222	223

6 h	15.30	15.35	15.26	15.28	15.21	15.21	15.22	15.22	15.23	15.22	15.22	15.23
24 h	15.27f	15.27f	15.33^ef	15.46^cd	15.41^de	15.47^cd	15.43^de	15.46^cd	15.44^cde	15.77^a	15.56^bc	15.59^b
48 h	15.97^ab	15.95^ab	15.96^ab	15.98^ab	15.97^ab	15.92^ab	15.99^ab	15.81^b	16.02^a	15.84^ab	15.85^ab	15.85^ab

Time	Valerate

	111	112	113	121	122	123	211	212	213	221	222	223

6 h	15.24^ab	15.25^ab	15.20^ab	15.20^ab	15.16^b	15.18^ab	15.16^b	15.18^ab	15.18^ab	15.16^b	15.17^ab	15.39^a
24 h	15.20^c	15.21^c	15.20^c	15.31^bc	15.24^bc	15.32^bc	15.32^abc	15.30^abc	15.32^abc	15.58^a	15.45^a	15.41^a
48 h	15.74	15.71	15.73	15.74	15.74	15.68	15.71	15.58	15.72	15.61	15.61	15.61

111=RDP 60%, NFC 35%, Sulfur 0%; 112=RDP 60%, NFC 35%, Sulfur 0.15%; 113=RDP 60%, NFC 35%, Sulfur 0.3%; 121=RDP 60%, NFC 40%, Sulfur 0%; 122=RDP 60%, NFC 40%, Sulfur 0.15%; 123=RDP 60%, NFC 40%, Sulfur 0.3%; 211=RDP 65%, NFC 35%, Sulfur 0%; 212=RDP 65%, NFC 35%, Sulfur 0.15%; 213=RDP 65%, NFC 35%, Sulfur 0.3%; 221=RDP 65%, NFC 40%, Sulfur 0%; 222=RDP 65%, NFC 40%, Sulfur 0.15%; 223=RDP 65%, NFC 40%, Sulfur 0.3%, VFA=Volatile fatty acids, NFC=Non-fiber carbohydrate, RDP=Rumen degradable protein, Superscript ^a,b,c,d,e,fin different parameter and observation time means significantly different (p > 0.05)