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. Author manuscript; available in PMC: 2024 May 3.
Published in final edited form as: Nat Chem Biol. 2023 Oct 26;20(5):594–604. doi: 10.1038/s41589-023-01460-w

Extended Data Fig. 3.

Extended Data Fig. 3.

Oxygen consumption rate (OCR) (a) and extracellular acidification rate (ECAR) (b) of HeLa cells expressing EcSTH and mitoEcSTH before and after addition of 1 μM antimycin A (ANT) measured in pyruvate free HEPES/DMEM+dFBS media. Values are mean ± s.d.; n = 12, 12, 12, 10 for OCR traces in (a), n = 4 for bar graphs depicting OCR quantification in (a), n = 12, 12, 12, 10 for ECAR tracers in (b), n = 4 for bar graphs depicting ECAR quantification in (b) biologically independent samples. The statistical significance indicated for (a-b) represents a One-Way ANOVA followed by uncorrected Fisher’s least significant difference test.