Fig. 2.

Effects of interleukin 33 (IL-33) in SCC25 and Detroit 562 cells. (a) Relative wound density curve of SCC25 cells for the IncuCyte analysis over 24 h. Representative images of scratch assays show scratches immediately after they were made (0 h) and after 24 h in the presence of IL-33 (right panels) versus positive control medium (left panels). The scale bars represent 300 μm. (b) Proliferation curve for SCC25 cells in the presence of IL-33. Representative images of the proliferation assay. (c) Relative wound density curve of Detroit 562 cells for the IncuCyte analysis over 24 h. Representative images of scratch assays show scratches immediately after they were made (0 h) and after 24 h in the presence of IL-33 (right panels) versus positive control medium (left panels). The scale bars represent 300 μm. (d) Proliferation curve for Detroit 562 cells in the presence of IL-33. Representative images of the proliferation assay. The data are shown as the mean ± standard error of the mean (SEM) from a single experiment and are representative of at least three experiments. (e) Transwell invasion assay for SCC25 cells at 48 h after incubation with IL-33 (10, 50 and 100 ng/ml). The data are shown as the mean ± SEM of cells counted in five representative microscopic fields per membrane and analysed using the ImageJ software. Representative images of invasion assay from two experiments. (f) Relative expression of EPCAM, SOX2, NANOG, MYC, EZH2, CHGA, AURKA, MYCN and SYP in SCC25 cells determined with quantitative polymerase chain reaction (mean ± SEM, n = 3). (g) Relative expression of EPCAM, SOX2, NANOG, MYC, EZH2, CHGA, AURKA, MYCN and SYP in Detroit 562 cells determined with quantitative polymerase chain reaction (mean ± SEM, n = 3). *P < 0.05, (**P < 0.01), ***P < 0.001 and ****P < 0.0001